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1. This phenomenon is called clotting or blood coagulation. On further maintaining, the clot retracts into a smaller volume and presses a clear straw coloured fluid, called the serum. Serum won’t clot anymore. When the practice of blood coagulation is analyzed under the microscope, it is seen that, moment granules appear initially, often near a clump of disintegrating platelets. These granules combine together to form needles, which unite with each other to form long threads across the entire bulk of blood. These threads cross one another and produce a kind of network, to the meshes where the red and white cells eventually become entangled.
The clot progressively retracts and serum separates out. It’s to be noted that bloodstream coagulation is the property of plasma alone. The white and red cells don’t take part in it. They only eventually become caught up in the meshes of the clot and are thus removed. Blood platelets take some part in the practice. Normal Coagulation Time: Measured in accordance with the method of Lee and White it’s 6 into 17 minutes in glass tube and 19 into sixty minutes in siliconised tube. Essay. 2. Methods Determining Blood Coagulation: i. Capillary Glass Tube Method: This method is usually adopted as a bed side procedure.
The finger is dotted and the bloodstream is made to flow in a capillary glass tube about 15 cm long. A small bit of the glass tube is cautiously broken off every 15 seconds until a fine thread of clotted bloodstream appears whilst the tube is being broken. The period between the appearance of bloodstream in the finger and the formation of the thread is taken as the bloodstream coagulation time. The average time, by this method, is 3-4 minutes. Ii. Wrights Coagulometer: The principle is same as above. Blood is allowed to flow to a dozen bronchial tubes of equal calibre. The tubes are sealed on both sides and placed in water bath at 37C. After 4 minutes, the first tube is removed from the water bath, the ends are broken and the bloodstream inside is ejected into water.